Benjamin Stewart

Contact information

Lawrence Livermore National Laboratory

7000 East Avenue, L-452

Livermore, CA 94550

Email: stewart66@llnl.gov

phone: 925 424-2819

  

Education

Post-Doctoral Researcher, Lawrence Livermore National Laboratory, 2009-2012

Ph.D., Toxicology, University of Colorado, 2008

B.S., Molecular Biology, Brigham Young University, 2001

Research interests

Analytical biochemistry, chromatography, mass spectrometry, quantitative metabolism.

 

My research primarily involves the use of accelerator mass spectrometry quantify 14C-labeled tracers in a variety of biological systems.  I am involved in characterization of metabolic fates of xenobiotics, measurement of post-translational and chemical modifications of proteins, and elucidation of metabolite exchange between microorganisms.   My work also involves development of analytical biochemistry methods and application of these methods to problems in basic biochemistry, pharmacology and toxicology.

 

 Publications:

1.            Tkavc, R.; Matrosova, V. Y.; Grichenko, O. E.; Gostinčar, C.; Volpe, R. P.; Klimenkova, P.; Gaidamakova, E. K.; Zhou, C. E.; Stewart, B. J.; Lyman, M. G.; Malfatti, S. A.; Rubinfeld, B.; Courtot, M.; Singh, J.; Dalgard, C. L.; Hamilton, T.; Frey, K. G.; Gunde-Cimerman, N.; Dugan, L.; Daly, M. J., Prospects for Fungal Bioremediation of Acidic Radioactive Waste Sites: Characterization and Genome Sequence of Rhodotorula taiwanensis MD1149. Frontiers in Microbiology 2018, 8 (2528).

2.            Gates, S. D.; Condit, R. C.; Moussatche, N.; Stewart, B. J.; Malkin, A. J.; Weber, P. K., High initial sputter rate found for Vaccinia virions using isotopic labeling, NanoSIMS and AFM. Anal Chem 2018.

3.            Abu Aboud, O.; Habib, S. L.; Trott, J.; Stewart, B.; Liang, S.; Chaudhari, A. J.; Sutcliffe, J.; Weiss, R. H., Glutamine Addiction in Kidney Cancer Suppresses Oxidative Stress and Can Be Exploited for Real-Time Imaging. Cancer Res 2017, 77 (23), 6746-6758.

4.            Trott, J. F.; Kim, J.; Aboud, O. A.; Wettersten, H.; Stewart, B.; Berryhill, G.; Uzal, F.; Hovey, R. C.; Chen, C. H.; Anderson, K.; Graef, A.; Sarver, A. L.; Modiano, J. F.; Weiss, R. H., Inhibiting tryptophan metabolism enhances interferon therapy in kidney cancer. Oncotarget 2016.

5.            Kim, J.; Stewart, B.; Weiss, R. H., Extraction and Quantification of Tryptophan and Kynurenine from Cultured Cells and Media Using a High Performance Liquid Chromatography (HPLC) System Equipped with an Ultra-Sensitive Diode Array Detector. Bio-protocol 2016, 6 (7).

6.            Mayali, X.; Stewart, B.; Mabery, S.; Weber, P. K., Temporal succession in carbon incorporation from macromolecules by particle-attached bacteria in marine microcosms. Environmental microbiology reports 2016, 8 (1), 68-75.

7.            Wettersten, H. I.; Hakimi, A. A.; Morin, D.; Bianchi, C.; Johnstone, M. E.; Donohoe, D. R.; Trott, J. F.; Aboud, O. A.; Stirdivant, S.; Neri, B.; Wolfert, R.; Stewart, B.; Perego, R.; Hsieh, J. J.; Weiss, R. H., Grade-Dependent Metabolic Reprogramming in Kidney Cancer Revealed by Combined Proteomics and Metabolomics Analysis. Cancer Res 2015, 75 (12), 2541-52.

8.            Stewart, B. J.; Navid, A.; Kulp, K. S.; Knaack, J. L.; Bench, G., D-Lactate production as a function of glucose metabolism in Saccharomyces cerevisiae. Yeast 2013, 30 (2), 81-91.

9.            Thomas, A. T.; Stewart, B. J.; Ognibene, T. J.; Turteltaub, K. W.; Bench, G., Directly Coupled High-Performance Liquid Chromatography–Accelerator Mass Spectrometry Measurement of Chemically Modified Protein and Peptides. Analytical Chemistry 2013, 85 (7), 3644-3650.

10.          Navid, A.; Ng, D.; Stewart, B.; Wong, S.; Lightstone, F., Quantitative In Silico analysis of transient metabolism of acetaminophen and associated causes of hepatotoxicity in humans. In Silico Pharmacology 2013, 1 (1), 14.

11.          Stewart, B. J.; Bench, G.; Buchholz, B. A.; Haack, K. W.; Malfatti, M. A.; Ognibene, T. J.; Turteltaub, K. W., Accelerator Mass Spectrometry in Pharmaceutical Development. In Mass Spectrometry Handbook, Lee, M. S., Ed. John Wiley & Sons, Inc.: Hoboken, New Jersey, 2012; pp 259-269.

12.          Jiao, Y.; Navid, A.; Stewart, B. J.; McKinlay, J. B.; Thelen, M. P.; Pett-Ridge, J., Syntrophic metabolism of a co-culture containing Clostridium cellulolyticum and Rhodopseudomonas palustris for hydrogen production. International Journal of Hydrogen Energy 2012, 37 (16), 11719-11726.

13.          Buchholz, B. A.; Sarachine Falso, M. J.; Stewart, B. J.; Haack, K. W.; Ognibene, T. J.; Bench, G.; Salazar Quintero, G. A.; Malfatti, M. A.; Kulp, K. S.; Turteltaub, K. W., Bioanalytics for Human Microdosing. In Encyclopedia of Drug Metabolism and Interactions, Lyubimov, A. V., Ed. John Wiley & Sons, Inc.: Hoboken, New Jersey, 2012.

14.          Ronis, M. J.; Hennings, L.; Stewart, B.; Basnakian, A. G.; Apostolov, E. O.; Albano, E.; Badger, T. M.; Petersen, D. R., Effects of long-term ethanol administration in a rat total enteral nutrition model of alcoholic liver disease. Am J Physiol Gastrointest Liver Physiol 2011, 300 (1), G109-19.

15.          Shearn, C. T.; Smathers, R. L.; Stewart, B. J.; Fritz, K. S.; Galligan, J. J.; Hail, N., Jr.; Petersen, D. R., Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) inhibition by 4-hydroxynonenal leads to increased Akt activation in hepatocytes. Mol Pharmacol 2011, 79 (6), 941-52.

16.          Smathers, R. L.; Galligan, J. J.; Stewart, B. J.; Petersen, D. R., Overview of lipid peroxidation products and hepatic protein modification in alcoholic liver disease. Chem Biol Interact 2011, 192 (1-2), 107-12.

17.          Stewart, B. J.; Navid, A.; Turteltaub, K. W.; Bench, G., Yeast dynamic metabolic flux measurement in nutrient-rich media by HPLC and accelerator mass spectrometry. Anal Chem 2010, 82 (23), 9812-7.

18.          Roede, J. R.; Stewart, B. J.; Petersen, D. R., 9.26 - Hepatotoxicity of Reactive Aldehydes. In Comprehensive Toxicology (Second Edition), McQueen, C. A., Ed. Elsevier: Oxford, 2010; pp 581-594.

19.          Sporty, J.; Lin, S. J.; Kato, M.; Ognibene, T.; Stewart, B.; Turteltaub, K.; Bench, G., Quantitation of NAD+ biosynthesis from the salvage pathway in Saccharomyces cerevisiae. Yeast 2009, 26 (7), 363-9.

20.          Stewart, B. J.; Roede, J. R.; Doorn, J. A.; Petersen, D. R., Lipid aldehyde-mediated cross-linking of apolipoprotein B-100 inhibits secretion from HepG2 cells. Biochimica et biophysica acta 2009, 1791 (8), 772-80.

21.          Roede, J. R.; Stewart, B. J.; Petersen, D. R., Decreased expression of peroxiredoxin 6 in a mouse model of ethanol consumption. Free Radic Biol Med 2008, 45 (11), 1551-8.

22.          Cheng, L.; Stewart, B. J.; You, Q.; Petersen, D. R.; Ware, J. A.; Piccotti, J. R.; Kawabata, T. T.; Ju, C., Covalent binding of the nitroso metabolite of sulfamethoxazole is important in induction of drug-specific T-cell responses in vivo. Mol Pharmacol 2008, 73 (6), 1769-75.

23.          Sampey, B. P.; Stewart, B. J.; Petersen, D. R., Ethanol-induced modulation of hepatocellular extracellular signal-regulated kinase-1/2 activity via 4-hydroxynonenal. J Biol Chem 2007, 282 (3), 1925-37.

24.          Stewart, B. J.; Doorn, J. A.; Petersen, D. R., Residue-specific adduction of tubulin by 4-hydroxynonenal and 4-oxononenal causes cross-linking and inhibits polymerization. Chemical research in toxicology 2007, 20 (8), 1111-9.

25.          Tao, Y.; Kupfer, R.; Stewart, B. J.; Williams-Skipp, C.; Crowell, C. K.; Patel, D. D.; Sain, S.; Scheinman, R. I., AIRE recruits multiple transcriptional components to specific genomic regions through tethering to nuclear matrix. Mol Immunol 2006, 43 (4), 335-45.